Shigowa da
● Tabbatar da hakar DNA
A cikin duniyar ƙwayoyin kwayoyin halitta, hakar halittar DNA itace ne na asali wanda ke sanya tushe don daidaitaccen tsarin aikace-aikace da kuma maganin cuta. Tsarin hakar halittar DNA ya ƙunshi ware DNA daga sel don bincika abubuwa na kwayoyin halitta, yana ba da rahusa mai mahimmanci cikin cututtukan ƙwayar cuta, da kuma ilimin juyin halitta, da ci gaban ilimin halittu. Tare da bukatar burging don karatun iri-iri, rawar da abin dogaroKit ɗin hawan galibin halittubai taba kasancewa mafi yawan mutane ba. Wadannan abubuwan da suka shafi tsari na hakar, tabbatar da yawan amfanin ƙasa da kuma tsarkakakkiyar DNA, mai mahimmanci ga aikace-aikacen ƙasa.
Samfuran tattara tarin
● Yawancin samfuran don hakar DNA
Ingancin halittar halittar dan adam yana farawa da tarin babban aiki - samfuran inganci. Hanyoyin gama gari sun haɗa da jini, nama, yau, da Bucucal swabs. Kowane nau'in samfurin yana ba da fa'idodi na musamman; Misali, samfuran jini suna da arziki a cikin DNA amma yana buƙatar kulawa da kulawa, yayin da Siva da swabs swabs suna ba da zaɓuɓɓuka waɗanda ba za zaɓi ba. Zabi samfurin da ya dace yana da mahimmanci, musamman lokacin amfani da kayan haɓakar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayoyin cuta, wanda zai tabbatar da yawan amfanin sel, mai tsabta.
● Mafi kyawun ayyukan don sarrafa samfurin
Tabbatar da amincin Ma'anar yana da mahimmanci ga nasarar DNA. Dole ne a tattara samfurori a cikin gurbatawa - Yanayin kyauta, ana adana shi a yanayin da ya dace, kuma yana kulawa da kulawa don hana lalata. Wannan matakin yana da mahimmanci ko kuna ma'amala da sabbin samfurori ko waɗanda aka adana na dogon lokaci, kamar yadda duk mai lalata zai iya magance yawan amfanin ƙasa na ƙarshe da inganci, yana shafar sakamako na gwaji.
Hanyar Lysel
● Chememer vs. jiki Lysis dabaru
Mataki na tantanin halitta shine pivotal wajen sakin kwayar halittar dabbobi daga tsarin salula. Cinta Lysis, wanda ya shafi wanka da enzymes, a hankali yana rushe membranes da sunadarai. Hanyoyi na zahiri - kamar rushewar injin da sonication - sun fi ƙarfin zuciya kuma ana iya amfani dashi don samfuran Tougher. Zaɓin hanya sau da yawa ya dogara da nau'in samfurin da takamaiman buƙatun na kayan haɓaka DNA a cikin amfani. Masu kera kayayyaki don inganta ingancin Lysis, daidaita tsakanin sauki da tasiri.
● Muhimmancin lysis a cikin DNA Sakin
Ingancin Lysis ta tabbatar da cewa an warware kayan aikin salula sosai, yana sauƙaƙe sakin m cikin mafita. Daidai da yadda ya kamata a kashe ta DNA da kuma gurbatawa daga sunadarai da lipids, wanda yake musamman da aikace-aikacen tantanin halitta, inda amincin ƙuji yake.
Cire sunadarai da impurities
● Matsakaicin abubuwan da aka tsarkake cikin tsarkakewa
Post - Lysis, cirewararren sunadarai da sauran ƙazanta suna da mahimmanci. Hanyoyin da ake so kamar Phenol - ana daukar chlorioform da keɓaɓɓe da kuma raba sunadarai daga nucleic acid. Abincin halittar DNOm na zamani suna amfani da silica - tushen membranes ko beads na Magnetic, wanda ke da hankali DNa zaɓi. Wannan matakin yana da mahimmanci wajen samun DNA tsarkakakke don madaidaici aikace-aikace da mai hankali ƙasa.
● Centrifugation ya bayyana
Centrifugation shine matakin gama gari don rarrabe DNA daga post na impurities - lysis. Ta hanyar amfani da karfin centrifugal, tarkace mai nauyi da sunadarai sun fi kyau, yayin da DNA ke nan cikin sanannun. Wannan matakin galibi ana maimaita wannan matakin a cikin Tandem tare da matakan tsarkakewa don haɓaka tsarkakakku da kuma maida hankali ga dNA.
Tsarin DNA
● Amfani da ISOPropanol da ethanol
Yanayi shine hanya mai ma'ana don DNA daga mafita, ana cinyewa a kusan dukkanin kayan girke-girke na DNA. Barasa kamar Itopropanol da ethanol ya ba da DNA don yin hazo a kan rage karancin sa. Da zarar an procipitated, DNA ya canza bayyane clumps ko filayoyi, suna ba da haɗin haɗi na ci gaba da nasara.
● Abubuwan haɗin DNA Fastals
Kasancewar filayen DNA na tabbatar da ingantaccen hazo kuma yana aiki a matsayin farkon nasarar hakar. Wannan matakin, ko da yake ga alama na asali ne, yana da mahimmanci don amincewa da tsarin hakar, tabbatar da cewa isasshen DNA ya kasance don bincike mai zuwa.
Wanke da DNA
● Muhimmancin wankin ethanol
Wanke dubts da ethanol yana aiki don cire kayan salts da impurities. Wannan matakin, kodayake yana da alama na yau da kullun, yana da mahimmanci wajen hana gurbatawa wanda zai iya shafar tafiyar matakai na ƙasa. Wanke Wanke da ta dace a cikin kowane Manual na mai amfani ta hanyar haɓakar kitsenan wasan na DNA don tabbatar da masu amfani da sakamako mafi kyau.
● tabbatar da tsabta na DNA
Tsarkake yana da mahimmanci kamar amfanin ƙasa a cikin hakar DNA. Residimy impurities na iya hana enzymatic halayen enzymatic a cikin saukar da aikace-aikacen ƙasa kamar muCR da sequincing. Masu samar da kayan girke-girke na dNA na kwayar halittu suna karfafa tsarki, suna bayar da ladabi da kayan da suka tabbatar da crabpleed sosai.
Narkewa da shirya DNA
● zabar hannun dama
Da zarar an tsarkake DNA, an narkar da shi a cikin buffer mai dacewa, yawanci se mai buffer ko ruwa mai narkewa. Zabi na mai buffer na iya shafar kwanciyar hankali da amincin DNA, su fi tsayi tsawon - uni na iya zama. Abubuwan haɓakawa na kayan masana'antu na kwayoyin halitta yawanci suna samar da buffers wanda aka tsara don takamaiman aikace-aikacen ƙasa.
● Shirya DNA don gwaje-gwajen
Shirye-shiryen DNA yana da mahimmanci ga nasarar gwaji, tabbatar da karfinsu tare da dabarun bincike kamar qpc, na gaba - tsararraki - kuma cloning. Hanyoyin shirye-shirye na shirye-shiryen don ƙara girman amincin DNA da aiki a cikin waɗannan aikace-aikacen.
Ingancin iko na cire DNA
● dabaru don tantance ingancin DNA
Bayan hakar, tantance ingancin DNA yana da mahimmanci. Spectrophotometry ne sanannen hanya, aunawa shedance a 260 nm da bayar da bayanai game da maida hankali game da daidaitawar DNA da kuma tsarkakakke. Bugu da ƙari, gel electrophoreseseses yana ba da damar gani na mutuntawa DNA, Gano duk da lalata.
● GASKIYA GASKIYA
Karatun Spectrophotometer suna ba da fahimta cikin taro da kuma tsarkakakken DNA. A 260/280 rabo kusa da 1.8 yana nuna tsabtace DNA, yayin karkata da ke ba da izini. Waɗannan karatun suna da mahimmanci wajen tabbatar da cewa DNA ta dace da aikace-aikacen ƙasa mai ƙarfi.
Kalubale a hakar DNA
Batutuwa na yau da kullun da matsala
Duk da ci gaba, kalubale a hirar DNA ya dage, ciki har da ƙarancin yawan amfanin ƙasa, gurbancewa, da lalata. Fahimtar waɗannan batutuwan da bin jagororin matsala-sau da yawa ana bayar da su daga masana'antun - yana da mahimmanci don hakar mai nasara.
● Inganci tare da nau'ikan samfuri daban-daban
Hanyoyin samfuri daban-daban suna nuna fifikon ƙalubale a cikin hakar DNA, irin su masu canzawa DNA abun ciki da kasancewar masu hana su. Kitunan kayan girke-girke na samar da kayan samar da kayan aikin don magance waɗannan bambance-bambancen, bayar da mafita ga samfuran halittu daban-daban.
Ci gaba a cikin fasahar hakar DNA
● Abubuwan da ake amfani da su a cikin kayan hakar
A ci gaba da cigaban cigaba a cikin hakar kayan masana'antu na iri-iri sun sauƙaƙa tsari, haɓaka ƙarfi, saurin, da aminci. Wadannan sababbin saben fasaha, da haɗin kai tare da robotics don aikace-aikacen kayan aiki na manyan kayan aiki, suna nuna yanayin binciken kwayoyin bincike.
● Za'a yi abubuwan da zasu faru a ilimin kimiyyar mallaka
A matsayin ƙimar ilimin ilimin kimiya na kwarai, buƙatun na sama - hakar DNA za ta yi girma. Abubuwan da zasu biyo baya sun hada da ƙarin ECO - Hanyoyin hakar abokantaka, da hadewar hakar tare da cikakkiyar bincike mai mahimmanci.
BlueKIT: majagaba a cikin samutions da mafita na salula
Jiangsu Hillagene, karkashin alamaAlishir, yana tsaye a farkon samarwa da kayan adon salula. Hedkwen kai a Suzhou tare da ƙarin wuraren aiki a Shenzhai da Shanghai, da kuma shafin mai zuwa a North Carolina, Hillgene yana haɓaka shi a duniya. Alishir®Products suna ba da cikakkun hanyoyin mafi inganci don iko mai inganci a cikin sel. Ta hanyar samar da kayan aikin masana'antu da QC, BlueKIT yana jujjuyawa yanayin yanayin warkarwa, yana kawo samfuran canzawa zuwa kasuwa da sauri kuma mafi inganci.
Lokaci: 2024 - 12 - 05 15:07:02
