Menene fa'idodin amfani da Kit ɗin BCA?

Gabatarwa zuwa Asarar furotin Assayi

BCA (Bicinchoninic acid) protin peryay ne mai mahimmanci dabaru a cikin ainihin binciken biochemical da maganin ƙwayoyin cuta. Yana sauƙaƙe ƙididdigar jimlar furotin na tattarawa a cikin samfurin. Wannan hanyar tana aiki akan ka'idar furote - jan ƙarfe Chaier, wanda aka gano ta hanyar gano launi na rage ion ions. Ya shahara mai tushe daga yawan aiki a duk faɗin samfuran furotin da yawa, gami da ƙari ga masu kera, waɗanda ke da zaɓaɓɓen zaɓaɓɓu don masu kera da masu kaya a fagen.

Dacewa da kayan wanka

TAFIYA TAFIYA TAFIYA

Ofaya daga cikin abubuwan da aka sani na abubuwan da aka sani na BCA furotin assay shine daidaitonsa tare da kayan wanka. Ba kamar sauran hanyoyin da yawa na furotin ba, assulsay zai iya ɗaukar tsayayye har zuwa Surfactants 5% a cikin samfurin. Wannan fasalin yana da amfani ga masu bincike da masana'antun da ke ma'amala da samfurori, inda galibi ana amfani da kayan abinci don warwarewa. Don aikace-aikacen warkarwa na tantanin halitta, wannan dacewa yana tabbatar da cewa sikelin furotin ya kasance daidai duk da kasancewar waɗannan abubuwa masu shiga tsakani.

Yi cikin kalubale

Bca Assay tana ba da ingantacciyar sakamako koda a cikin kalubalantar yanayin gwaji. Haɗinta game da bambancin furotin, kamar sarƙoƙi na amino acid, yana rage furotin - don - Protin Presia. Wannan daidaituwa yana da mahimmanci ga masu siyar da masu ba da izini da kuma sakamakon haifuwa don kula da babban - ƙa'idodin inganci a cikin furotin.

Rage lokacin saiti

Inganci tare da dilowe - ma'aunai kyauta

Gabatarwa da diloorewa - Matsayi na furotin kyauta a cikin kit ɗin BCA na Assul a lokacin da assay saita assay zuwa kashi 80%. Wadannan ka'idojin da aka annabta an tsara su ne don hadewa mara kyau tare da butettes na multichann, masu ba da damar masu bincike da masana'antu don fitar da tsarin daidaito ba tare da daidaita daidaito ba. Don Aikace-aikacen Tsarin Cely, inda lokaci - Gwaji mai mahimmanci sun zama ruwan dare gama gari, wannan Ingancin yana da mahimmanci.

Sauƙaƙe aiki

Wannan hanyar da aka daidaita ta rage buƙatar matakan dake jagorancin jagora, wanda ba lokaci bane kawai. Ta hanyar kawar da waɗannan matakan, BCA Assay yana sauƙaƙe wani juye-yare na sauri, kyale masu ba da izinin saduwa da jadawalin isarwa da haɓaka yawan aiki a cikin saitunan ɗakin bincike.

Ingantaccen daidaitaccen abinci

Cikakken ma'auni a cikin nau'ikan furotin

Barka da Assul Assay Nuna karin martani ga sunadarai daban-daban idan aka kwatanta da na dye - da karfi da assays kamar hanyar Bradford. Wannan daidaitaccen tsari ana samunsa ta hanyar amsawa zuwa yawan shaidan peptide, rage girman tunanin assay ga bambance-bambance a cikin tsarin furotin. Ga masana'antun da ke tattare da samar da maganin shan giya, wannan na tabbatar da cikakken auna a cikin tsarin furotin daban-daban, inganta amincewa da sakamakon assay.

Ingantaccen daidaitawa

Ta hanyar samar da rashin daidaito ba tare da la'akari da bambancin furotin ba, Assa assay shine mahalli ga masu kaya don buƙatar adadi daidai don daidaitaccen iko da daidaitaccen samfurin. Wannan daidaiton ya fassara zuwa mafi amintattun bayanai, mahimmanci don ci gaba da bincike mai mahimmanci a cikin ilimin tantanin halitta da filayen da suka danganta.

Jin daɗin ganowa da kewayon ganowa

Babban hankali ga ƙananan taro

Kungiyar BCA Assay tana bayar da babban matakin hankali, wanda zai iya gano girman furotin kamar yadda 0.5 μg / mg / ml. Wannan kewayon babban iyaka yana sa ya dace da aikace-aikace iri-iri, daga bincike na asali don ci gaba da ci gaban warkewa. Irin wannan tunanin yana da mahimmanci yayin ma'amala da adadin adadin sunadarai a cikin ƙwayar sel da magunguna.

Mafi kyau duka sigogi

Gano launi na launi a 562 NM yana tabbatar da asarar siginar ƙasa (ƙasa da 10%), ba masu kera masana'antu da masu ba da kuɗi don cimma daidaitaccen adadi. Wannan daidaitaccen abu ne mai mahimmanci don haɓaka samfuran kwayar halitta na kwayar halitta, inda tsaka-tsakin furotin maida hankali ne na iya tasiri kai tsaye tasiri na warkewa.

Sauƙin Amfani da Tsarin Assay

Hanyoyin da aka jera

Kungiyar BCA Assay fasali na sauƙaƙe hanyoyin, da amfani ga sababbin masu amfani da ƙwararrun masu samarwa. A shirye - zuwa - Yi amfani da reagents da madaidaiciyar yarjejeniya da kuma masu samar da masu sauraro, gami da masu masana'antu da masu kaya don sulhu a kan hanyoyin furotin.

Mai amfani - Tsarin abokantaka

An tsara shi da sauƙi na amfani da shi, BCA Assay tana rage wahalar Fasaha, Masu sauƙaƙe horo da sauri tallafi a cikin saitin dakin gwaje-gwaje. Wannan damar dama tana taimakawa ayyukan layinta don masu ba da izini waɗanda suke buƙatar kula da babban kayan fitarwa ba tare da tsara daidaito ba.

Daidaito da daidaito

Amintaccen sakamako a duk faɗin gwaje-gwaje

Sakamakon amintaccen sakamako, wanda ya kamu da ƙarancin furotin - to - Bambancin furotin. Wannan daidaiton yana da mahimmanci don gwaje-gwaje don gwaje-gwaje a kan lokaci, kamar waɗanda ke cikin ƙwayar cuta, inda ingantaccen sikelin furotin ya zama paramount don haifuwa da tabbataccen sakamako.

Daidaitawa da daidaitawa

Rashin daidaituwa game da tsarkakakkiyar BSA daga Cibiyar Halittar Kasa da Fasaha ta Kasa (NIR) ta tabbatar da cewa assayay daidaitattun abubuwa masu saurin fahimta. Wannan sauyin yana da mahimmanci ga masana'antun da masu samarwa suna nufin isar da High - inganci, samfurori masu daidaitawa.

Bangarorin fasaha na hanyar BCA

Kayan biochemical

Hanyar BCA ta dogara ne da rage matsalar CU²⁺to cu⁺Ta hanyar sunadarai a cikin alkalin alkaline, bi da samuwar wani hadaddun purple tare da bca, wanda ya zama mai nuna alama. Wannan mataki biyu - Mataki dauki yana ba da ingantaccen tushen tushen furotin, wanda aka zartar a cikin bincike daban-daban da masana'antu, gami da ci gaban kwastomomi.

Fa'idodi akan wasu maganganun

Idan aka kwatanta da hanyoyin kamar Bradford Asay, Hanyar BCA tana ba da mafi girma girma saboda yawan abubuwan shiga da daidaituwar ta a kan nau'ikan furotinsa daban-daban. Wannan ya sa ya fi so zabi ga masana'antun da masu ba da izini waɗanda suke buƙatar ingantattun hanyoyin kariya.

Kwatantawa da Bradford Assay

Bambancin bambance-bambance a Hanyar

Duk da yake duka biyun da Bradfords suna amfani da su sosai don ƙimar furotin, sun sha bamban da ke tsakanin su. The Bradford Assay Regory Regist Re a kan Dye - Girman furotin, wanda zai iya zama ya zama ya daidaita a cikin nau'ikan furotin daban-daban. Sabanin haka, Peptide na Peptide Bontay, wanda ke gano tushe yana ba da babban daidaituwa da aminci, musamman mai amfani ga masana'antu da masu siyarwa.

Abbuwan amfãni na bca assay

Yarda da BCA Assay tare da kayan wanka, fallasa tsauraran motsa jiki, kuma a sauƙaƙe aikin motsa jiki ba shi gefen aikace-aikacen da ke buƙatar waɗannan takamaiman ƙarfin. Abubuwan da ta fi dacewa da su dace da saitunan gwaji na gwaji, mahimmancin mahimmancin masu ba da kayayyaki daban-daban.

Kammalawa da shawarwari

Me yasa aka fi son BcA

Bca furotin assay yana tsaye don dacewa da kayan wanka, an rage yawan sashi, inganta furotin, da daidaito. Waɗannan halayen suna sanya shi sosai dacewa don aikace-aikace a cikin ƙwayar sel, ba da ingantaccen sakamako. Ga masana'anta da masu siyarwa, BCA Assayi yana ba da hanya mai dogaro don tabbatar da babban aiki da kuma mai mahimmanci don kiyaye ƙimar masana'antu da tallafawa ci gaban samfurin.

Bluekitit Sayar da mafita

Haske na Bluekitit ne a cikin bayar da mafita ga mafita ga BCA furotin Assay, wanda aka yiwa haduwa da takamammen bukatun masu binciken, masana'antun, da masu kaya. An tsara kits ɗinmu don ingantaccen aiki, samar da ingantacciyar ingantacciya da daidaitaccen ƙimar fursunoni a duk faɗin aikace-aikace. Tare da Bluekit, zaku iya haɓaka ingancin aikin ku da daidaito, tabbatar muku haɗuwa da mafi girman ƙa'idodi a cikin binciken da ke cikin tantanin halitta da ci gaba. Bari BlueChit ya zama abokin tarayya amintacciyar amana don duk abubuwan da aka furta.